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Plasmid#73839PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT316
Plasmid#8608DepositorInsertbarstar
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJK040 (pET-PurQ-A128T)
Plasmid#73840PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationA128T mutantPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT416
Plasmid#8607DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT413
Plasmid#8606DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJK034
Plasmid#72383PurposeProduces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)DepositorInsertphosphoribosylglycinamide synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK100
Plasmid#73264PurposeProduces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)DepositorInsertphosphoribosylformylglycinamidine synthase subunit PurS
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
StrepII-tag
Plasmid#55181PurposeStreptactin-tagDepositorInsertStrepII tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
His10-tag
Plasmid#55179Purpose10xHis-tagDepositorInsertHis10
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
cMyc-tag
Plasmid#55183PurposecMyc tagDepositorInsertcMyc tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-479.E152Q
Plasmid#17518DepositorInsertB. subtilis DEAD-box helicase YxiN
UseTagsintein-chitin binding domainExpressionBacterialMutationE152Q mutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PxylA
Plasmid#55178PurposeXylose-inducible promoterDepositorInsertxylA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterxylAAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PliaI
Plasmid#55177PurposeBacitracin-inducible promoterDepositorInsertliaI promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterliaIAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PliaG
Plasmid#55174PurposeConstitutive promoterDepositorInsertLiaG promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
J23101
Plasmid#55176PurposeVery weak constitutive promoterDepositorInsertJ23101 promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterJ23101Available sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only