We narrowed to 31,213 results for: ide

1. pAG1guide_RUBY_EggCas9

   Plasmid

   #225983

   Purpose

   To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

   Depositor

   Imran Siddiqi

   Insert

   Guide RNA against AtAGAMOUS1
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   35s promoter to Drive RUBY and DD45p to drive Cas9

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiGuide_mCherry_NT135

   Plasmid

   #183121

   Purpose

   pLentiGuide modified to express mCherry and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GuideVector_BbsI

   Plasmid

   #110692

   Purpose

   For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

   Depositor

   Douglas Mahoney

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiGuide_GFP_sgEPAS1#1

   Plasmid

   #183119

   Purpose

   pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiGuide_GFP_sgEPAS1#2

   Plasmid

   #183120

   Purpose

   pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EPAS1-2
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiGuide_mCherry_sgEPAS1#2

   Plasmid

   #183123

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-2
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLz74_Rubisco_CsoS2_Npeptide_fusion

   Plasmid

   #140840

   Purpose

   Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
   

   Tags

   CsoS2 N-peptide consensus to CbbL C-terminus and …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLR05_pGuide_B2M_sgRNA1

    Plasmid

    #131090

    Purpose

    sgRNA for SpCas9 mediated B2M KO

    Depositor

    Xiaojun Lian

    Insert

    B2M KO sgRNA 1 (B2M Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLR06_pGuide_B2M_sgRNA2

    Plasmid

    #131091

    Purpose

    sgRNA for SpCas9 mediated B2M KO

    Depositor

    Xiaojun Lian

    Insert

    B2M KO sgRNA 2 (B2M Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLz55_polyPro_random_Npeptide

    Plasmid

    #140846

    Purpose

    Same as pLz47 but with randomized N*-peptide sequence

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with randomized CsoS2 N-peptide
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-zeo

    Plasmid

    #160091

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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