We narrowed to 22,781 results for: Sis

1. PB-TAC-ERN

   Plasmid

   #80475

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-KIRIN1-GR

   Plasmid

   #107418

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-PEAR-mCherry

   Plasmid

   #228892

   Purpose

   All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Insert

   EGFP-IRES-mScarlet
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TAG-ERN

   Plasmid

   #80476

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. aCKAR

   Plasmid

   #124427

   Purpose

   FRET-based reporter for monitoring aPKC activity in cells

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

   Insert

   aCKAR
   

   Use

   Tags

   mCFP and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAG-ERP2

   Plasmid

   #80479

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-Trono-EFS-Blast-P2A-TurboRFP

   Plasmid

   #228893

   Purpose

   Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYESir2-puro

   Plasmid

   #1769

   Depositor

   Bob Weinberg

   Article

   Vaziri et al Cell 2001 Oct 19;107(2):149-59.

   Insert

   Sir2 (SIRT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rc/CMV cyclin C

   Plasmid

   #8961

   Depositor

   Bob Weinberg

   Article

   Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

   Insert

   cyclin C (CCNC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

10. BPTI-[5,55]Gly14Ile38

    Plasmid

    #195059

    Purpose

    Thermal stability of BPTI

    Depositor

    Yutaka Kuroda

    Article

    Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

    Insert

    bovine pancreatic trypsin inhibitor
    

    Use

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    Changed Alanine residues at position of 14 and 38…

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK-flag-USP19 C506S

    Plasmid

    #78584

    Purpose

    Expression flag-USP19 C506S in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 C506S (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 506 cysteine residue to serine

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

13. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pBS-KSTbx2.8.2

    Plasmid

    #42626

    Depositor

    Virginia Papaioannou

    Article

    Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

    Insert

    Tbx2 (Tbx2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-ND4 Right-GSVG-UGI

    Plasmid

    #187414

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-ND4 Right-E1347A-UGI

    Plasmid

    #187415

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK-flag-GFP 1-10 tetra Cys

    Plasmid

    #78589

    Purpose

    Expression flag-GFP 1-10 tetra Cys in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 1-10 tetra Cys
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-mPRAS40

    Plasmid

    #15480

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    PRAS40 shRNA (Akt1s1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Promoter

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCitrine-USP19 1-1290

    Plasmid

    #78595

    Purpose

    Express mCitrine-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only