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We narrowed to 944 results for: V2

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Showing: 381 - 400 of 944 results

  2. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  3. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  4. pPD301 ZF43x16-Compact_EYFP

    Plasmid
    #138911
    Purpose
    EYFP reporter vector for the ZF43x16-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x16-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x16-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138911-plasmid-map-sequence-id-324231

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  5. pPD299 ZF43x14-Compact_EYFP

    Plasmid
    #138909
    Purpose
    EYFP reporter vector for the ZF43x14-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x14-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x14-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138909-plasmid-map-sequence-id-324222

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  6. pPD294 ZF43x10-Compact_EYFP

    Plasmid
    #138905
    Purpose
    EYFP reporter vector for the ZF43x10-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x10-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x10-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138905-plasmid-map-sequence-id-324140

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  7. pPD300 ZF43x15-Compact_EYFP

    Plasmid
    #138910
    Purpose
    EYFP reporter vector for the ZF43x15-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x15-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x15-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138910-plasmid-map-sequence-id-324228

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  8. pPD298 ZF43x13-Compact_EYFP

    Plasmid
    #138908
    Purpose
    EYFP reporter vector for the ZF43x13-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x13-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x13-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138908-plasmid-map-sequence-id-324143

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  9. pPD295 ZF43x11-Compact_EYFP

    Plasmid
    #138906
    Purpose
    EYFP reporter vector for the ZF43x11-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x11-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x11-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138906-plasmid-map-sequence-id-324141

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  10. pPD293 ZF43x9-Compact_EYFP

    Plasmid
    #138904
    Purpose
    EYFP reporter vector for the ZF43x9-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x9-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x9-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138904-plasmid-map-sequence-id-324139

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  11. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Promoter
    EFS (P2A)
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187646-plasmid-map-sequence-id-367454

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  12. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

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  13. LentiRCas9-CUG

    Plasmid
    #104183
    Purpose
    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-CUGsgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104183-plasmid-map-sequence-id-201743

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  14. LentiRCas9-Lambda2

    Plasmid
    #104184
    Purpose
    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-sgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104184-plasmid-map-sequence-id-201745

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  15. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    TRE
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  16. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  17. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Promoter
    U6
    Available Since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  18. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  20. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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Showing: 381 - 400 of 944 results