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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,553 results for: golden gate

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Showing: 381 - 400 of 5553 results

  1. pICSL47772-LC

    Plasmid
    #245716
    Purpose
    Level 1 Position 5 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245716-plasmid-map-sequence-id-496045

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  2. pICSL47751-LC

    Plasmid
    #245714
    Purpose
    Level 1 Position 3 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245714-plasmid-map-sequence-id-496044

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  3. pICSL47732-LC

    Plasmid
    #245712
    Purpose
    Level 1 Position 1 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245712-plasmid-map-sequence-id-496042

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  4. pICSL47761-LC

    Plasmid
    #245715
    Purpose
    Level 1 Position 4 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245715-plasmid-map-sequence-id-496040

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  5. pICSL47781_RFP

    Plasmid
    #245703
    Purpose
    Level 1 Position 6 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245703-plasmid-map-sequence-id-496039

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  6. pICSL47772_RFP

    Plasmid
    #245702
    Purpose
    Level 1 Position 5 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245702-plasmid-map-sequence-id-496038

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  7. pICSL47761_RFP

    Plasmid
    #245701
    Purpose
    Level 1 Position 4 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245701-plasmid-map-sequence-id-496037

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  8. pICSL47742-LC

    Plasmid
    #245713
    Purpose
    Level 1 Position 2 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245713-plasmid-map-sequence-id-496035

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  13. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

    Plasmid
    #50624
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Mrna production
    Promoter
    T7
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50624-plasmid-map-sequence-id-75688

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  18. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  19. pICSL70008

    Plasmid
    #50336
    Depositor
    Nicola Patron
    Publication
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    Use
    Plant expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50336-plasmid-map-sequence-id-499941

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Showing: 381 - 400 of 5553 results