We narrowed to 559 results for: mak
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Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pMSCVII-mChe
Plasmid#162753PurposeRetroviral (MSCV) expression of mCherry fluorescent proteinDepositorInsertmCherry
UseRetroviralExpressionMammalianAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4137387
Plasmid#149544PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationwt;19-aa CtruncPromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV
Plasmid#102421PurposeInducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vectorDepositorInsertTet1 (Tet1 Mouse)
TagsHAExpressionMammalianMutationModified at the Dharmacon SMARTpool siRNA #2 targ…PromoterTetO-CMVAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 k46I
Plasmid#27624DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseGateway CloningTagsmycMutationInitial M from ULK1 removed. Silent mutation at A…Available sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
TagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …Available sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-eDHFR
Plasmid#176201PurposeNegative control of targeted optogenetic manipulation using TMP-Halo dimerization systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-eDHFR
ExpressionMammalianAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV
Plasmid#102422PurposeInducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vectorDepositorInsertTet1 catalytic domain mutant (Tet1 Mouse)
TagsHAExpressionMammalianMutationH1620Y and D1622A mutations in Tet1 catalytic dom…PromoterTetO-CMVAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM427
Plasmid#210788PurposeL5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transferDepositorInsertDefective hygromycin resistant gene
ExpressionBacterialMutationChanged hygromycin resistant gene W190 codon (TGG…Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)
Plasmid#39859DepositorAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
HOXA1 (murine) HIS-tag pET
Plasmid#8556DepositorInsertHOXA1 (Hoxa1 Mouse)
TagsHis and T7ExpressionBacterialMutationclone contains 130 AA including the homeodomain, …Available sinceJuly 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C2.0
Plasmid#99886PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU6Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ132C2.0
Plasmid#99889PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HOXB3 (human) HIS-tag pET
Plasmid#8524DepositorInsertHOXB3 (HOXB3 Human)
TagsHis and T7ExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HOXB4 (human) HIS-tag pET new RBS
Plasmid#8525DepositorInsertHOXB4 (HOXB4 Human)
TagsHis and T7ExpressionBacterialMutationContains improved Ribosomal binding site; makes …Available sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-LO
Plasmid#162754PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent proteinDepositorInsertLSSmOrange
UseRetroviralExpressionMammalianAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-AM
Plasmid#162751PurposeRetroviral (MSCV) expression of ametrine fluorescent proteinDepositorInsertametrine
UseRetroviralExpressionMammalianAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…Promotermyo6bAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT
Plasmid#163643PurposeMakes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.DepositorInsertsUseLentiviralTagsLuc2-P2A-tdTomato-T2AMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterEFS and pU6Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-N289A-H290A
Plasmid#82032PurposePlasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
TagsHAExpressionMammalianMutationEndonuclease-dead mutant: N289A+H290A CtIP; Mutat…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only