We narrowed to 8,954 results for: mel

1. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

   Plasmid

   #170240

   Purpose

   Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZLblasti-HA-MEK1

   Plasmid

   #53161

   Purpose

   Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGEX6P1-HA-MEK1 C121S

   Plasmid

   #53164

   Purpose

   Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   C121S

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGEX6P1-HA-MEK1 CBM

   Plasmid

   #53163

   Purpose

   Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   M187A/H188A/M230A/H239A

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pWZLblasti-HA-MEK1-MEK5DD

   Plasmid

   #53177

   Purpose

   Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET28A-Rab8A

   Plasmid

   #228880

   Purpose

   Expression of His-tagged Rab8A in E. coli

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   RAB8A (RAB8A Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   residues 1-6 and 177-207 missing

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pRSFDuet-1 IntS6 AA 1035-1284

   Plasmid

   #196904

   Purpose

   Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 6 (IntS6 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hy_pMT Laccase2 Exons 1-3

    Plasmid

    #91799

    Purpose

    Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1 IntS11 AA 300-597

    Plasmid

    #199329

    Purpose

    Expresses His-tagged Drosophila IntS11 amino acids 300-597

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 11 (IntS11 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 300-597 only

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYF-PRSx8-AstR-p2A-GFP

    Plasmid

    #159632

    Purpose

    Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

    Depositor

    Andrew Allen

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    AstR (AstA-R1 Fly)
    

    Use

    Lentiviral

    Tags

    GFP and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    PRSx8

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69899

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (stw Fly, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-EGFP-DmNot1_J

    Plasmid

    #146709

    Purpose

    Insect Expression of DmNot1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmNot1 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Three non silent mutations N605S, K759M, G1999S a…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KHBD00019

    Plasmid

    #34181

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5461 (bun Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00675

    Plasmid

    #39723

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6883 (trh Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    changed 478 Tyrosine for Alanine

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMtnA FLAG-IntS7 puro

    Plasmid

    #208405

    Purpose

    Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    pMT FLAG MCS puro (defl Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMtnA FLAG-IntS13 puro

    Plasmid

    #208406

    Purpose

    Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    IntS13 (asun Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMtnA FLAG-IntS14 puro

    Plasmid

    #208407

    Purpose

    Expresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    IntS14 (IntS14 Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge