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We narrowed to 902 results for: pgem

Showing: 381 - 400 of 902 results

  2. pGem-LP1-loxP-bar-lox2272-71

    Plasmid
    #168785
    Purpose
    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168785-plasmid-map-sequence-id-350000

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  3. pGem-LP2-loxP-bar-lox2272-71

    Plasmid
    #168786
    Purpose
    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168786-plasmid-map-sequence-id-353782

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  6. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid
    #119865
    Purpose
    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    2x myristoylation
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119865-plasmid-map-sequence-id-239419

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  9. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  10. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Expression
    Mutation
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

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  11. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

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  12. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  13. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Plasmid
    #224755
    Purpose
    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    In vitro transcription for oocyte expression
    Tags
    Expression
    Mutation
    S131C/C159A/C219A/C365A/C399A
    Promoter
    T7
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224755-plasmid-map-sequence-id-445090

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  14. pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Plasmid
    #224756
    Purpose
    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    In vitro transcription for oocyte expression
    Tags
    Expression
    Mutation
    S131A/C159A/C219A/C365A/C399A
    Promoter
    T7
    Available since
    Sept. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    224756-plasmid-map-sequence-id-445023

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  15. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Expression
    Mutation
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  16. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  17. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid
    #89890
    Purpose
    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    HA-BirA-2A-citrine
    Use
    Unspecified
    Tags
    Citrine
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89890-plasmid-map-sequence-id-181309

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Showing: 381 - 400 of 902 results