We narrowed to 5,973 results for: SPI

1. pCB004

   Plasmid

   #134147

   Purpose

   Bacterial expression of human Ska3

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   human Ska3 (SKA3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScalps Luciferase Zsgreen

   Plasmid

   #184784

   Purpose

   Lentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoter

   Depositor

   Ajit Chande

   Article

   Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

   Insert

   Firefly Luciferase and Zsgreen
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-U6+27-Tornado-RhoBAST-MS2

   Plasmid

   #196342

   Purpose

   Overexpression of circular RhoBAST-MS2

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-RhoBAST-MS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C2 RanGAP

   Plasmid

   #13378

   Depositor

   Mary Dasso

   Article

   Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

   Insert

   RanGAP (RANGAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His-SCov2-nsp7

   Plasmid

   #154757

   Purpose

   Expression of SARS-CoV-2 nsp7

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-puro-eGFP-HEC1

   Plasmid

   #114022

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1/HisC-mTAZ

   Plasmid

   #31793

   Depositor

   Michael Yaffe

   Article

   Hong et al Science. 2005 Aug 12;309(5737):1074-8.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His, and Xpre…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1 beta-catenin Y654F

    Plasmid

    #16072

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y654F, *(see below)

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046899

    Plasmid

    #145608

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. FCK-Halo-GFP

    Plasmid

    #14750

    Purpose

    Lentiviral expression of Halo-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al PLoS One. 2007 Mar 21;2(3):e299.

    Insert

    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N. pharaonis halorhodopsin is mammalian codon-opt…

    Promoter

    CaMKIIa

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. H6hSDHA

    Plasmid

    #113045

    Purpose

    Hetero expression of His-tagged human SDHA

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    H6hSDHA (SDHA Human)
    

    Use

    Tags

    His6 (MGSSHHHHHHSQDP-)

    Expression

    Bacterial

    Mutation

    Insert begins at amino acid 43

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Use

    Tags

    haemagglutinin

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMSCV-IRES-GFP-mouse-Caspase-11

    Plasmid

    #183358

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (Casp4 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-Brn2

    Plasmid

    #32925

    Purpose

    Retroviral expression of mouse Brn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

19. CAG_mCh_p85a_mCh

    Plasmid

    #199630

    Purpose

    FRET acceptor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    p85a-SH2 (Pik3r1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6-PRC1

    Plasmid

    #69111

    Purpose

    For bacterial expression of recombinant human PRC1 tagged with His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Protein Regulator of Cytokinesis 1 (PRC1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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