We narrowed to 4,240 results for: erf

1. TRP1MX6-ins4

   Plasmid

   #195041

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   TRP1
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN-hTRF2-tagRFP-T

   Plasmid

   #103812

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hTRF2: deletion of amino acids 1-42 and 476 compa…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. his3MX6-ins3

   Plasmid

   #195040

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   His5+
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCCL-PGK-SPdCas9-BFP-DNMT3B

   Plasmid

   #66820

   Purpose

   dCas9 fused to BFP and the human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
   

   Use

   Tags

   dSpCas9-BFP-DNMT3B

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. -53 bp human IFN-gamma luc

   Plasmid

   #17597

   Depositor

   Howard Young

   Article

   Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

   Insert

   IFN gamma promoter (IFNG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   -53 to +70 of promoter

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

   Plasmid

   #71214

   Purpose

   dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
   

   Use

   Tags

   dSpCas9-BFP-DNMT3B(E697A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139095

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

   Plasmid

   #71219

   Purpose

   dCas9 fused to the human DNMT3B catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3B(E697A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188770

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Islr2.b-Fc-His

    Plasmid

    #72077

    Purpose

    Expresses the extracellular region of the Islr2.b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Islr2.b (Islr2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Islr2.b-AP-His

    Plasmid

    #71951

    Purpose

    Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Islr2.b (Islr2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Jam4.1-Fc-His

    Plasmid

    #72081

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.1 (Igsf5 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mEGFP-hCTNNA1_L436P

    Plasmid

    #234559

    Purpose

    CTNNA1 eye phenotype via forward genetic screen

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_L436P (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    L436P

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSAVED-CHAT-sm_PCaspase

    Plasmid

    #214048

    Purpose

    Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS-BCD-Strep-SAVED-CHAT-sm

    Plasmid

    #214033

    Purpose

    Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. kanMX6-ins1

    Plasmid

    #195038

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_SEPT9_i1 R10A R15A-msfGFP

    Plasmid

    #180336

    Purpose

    mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R10A R15A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_SEPT9_i1 S12A S13A-msfGFP

    Plasmid

    #180337

    Purpose

    mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S12A S13A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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