We narrowed to 166,040 results for: addgene

1. NDUFV3_pCSdest

   Plasmid

   #53889

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   NDUFV3 (NDUFV3 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-tdPCP-GFP

   Plasmid

   #183934

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab32b

   Plasmid

   #140885

   Purpose

   Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab32b (rab32b Zebrafish)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSynWSyt1C2AB(80-421)msGFP

   Plasmid

   #209014

   Purpose

   Overexpression of truncated syt1 tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 80-421 only

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. B52_puro_empty_gRNA

   Plasmid

   #197557

   Purpose

   sites for cloning two gRNA with puromycin cassette

   Depositor

   Darius Bägli

   Type

   Empty backbone

   Use

   Empty grna backbone with puromycin resistance gene

   Expression

   Mammalian

   Promoter

   U6 for gRNA, CMV for puromycin resistance.

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPerimCh

   Plasmid

   #227013

   Purpose

   Expresses periplasmic mCherry

   Depositor

   Suzan Rooijakkers

   Article

   Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

   Insert

   PelB-mCherry, arabinose inducible cytosolic GFP
   

   Expression

   Bacterial

   Promoter

   rpsM and pBAD

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_C_PDK1

   Plasmid

   #111672

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PDK1 (PDK1 Human)
   

   Expression

   Mammalian

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Expression

    Mammalian

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMB3-B-TelR

    Plasmid

    #204992

    Purpose

    Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-eDIO-EYFP

    Plasmid

    #104388

    Purpose

    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

    Depositor

    Volker Busskamp

    Article

    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

    Insert

    Enhanced Yellow Fluorescent Protein
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 RagA shRNA #1

    Plasmid

    #30319

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCK364.306

    Plasmid

    #192642

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MG-155

    Plasmid

    #26529

    Depositor

    David Baltimore

    Article

    O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

    Insert

    miR-155 (MIR155 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. mc2 puro EF1a

    Plasmid

    #206221

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 RagA shRNA #2

    Plasmid

    #30320

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pRRL WS1.6 WASp

    Plasmid

    #36250

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Promoter

    WS1.6

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROSA26-FlpERT2

    Plasmid

    #149438

    Purpose

    ROSA26 mouse targeting vector with tamoxifen-inducible Flp recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Flp-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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