Addgene

We narrowed to 6,994 results for: Cad

Showing: 6031 - 6060 of 6994 results

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pcDNA3.1_hE2F7mg_115mut

Plasmid

#73077

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, m…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Human Wild-type ASAP1

Plasmid

#235212

Purpose

Expresses Wild-Type ASAP1 in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al Proc Natl Acad Sci U S A. 2018 May 1;115

Insert

ASAP1 (ASAP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_R2L2

Plasmid

#188049

Purpose

Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated R337L…

Promoter

T7

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_deltaAlpha4

Plasmid

#188050

Purpose

Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 362-452

Promoter

T7

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-ERG_DBD(306-429_F392A)

Plasmid

#188051

Purpose

Expresses His8-ERG_DBD(306-429_F392A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

ERG (ERG Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-305, 430-479, mutated F392A

Promoter

T7

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_F362A)

Plasmid

#188047

Purpose

Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated F362A

Promoter

T7

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-hei

Plasmid

#234485

Purpose

Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

myc and oNLS

Expression

Mutation

Promoter

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Plasmid

#220613

Purpose

Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only
pAAV-U6-sgPet-1-hSyn-mCherry-KASH

Plasmid

#223227

Purpose

guideRNA targeting the mouse Pet-1 (Fev)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Fev (Fev Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut1-Luc

Plasmid

#223664

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 1

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut2-Luc

Plasmid

#223665

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 2

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut3-Luc

Plasmid

#223666

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 3

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut4-Luc

Plasmid

#223667

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 4

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

Plasmid

#220614

Purpose

Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet

Use

AAV and Cre/Lox

Tags

3x HA; ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT-cbm

Plasmid

#214032

Purpose

Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

K121E, Q122A, N274A, R276E, Y285A, F286A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-dPCaspase

Plasmid

#214035

Purpose

Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

H79A, C146A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase-R153A

Plasmid

#214036

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

R153A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-BCL9

Plasmid

#202662

Purpose

Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-1

Plasmid

#202447

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET-hMICU1EF

Plasmid

#199180

Purpose

This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

Depositor

Ming-Feng Tsai

Article

Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

MICU1 (MICU1 Human)

Use

Tags

His6 and MBP, Thrombin site, TEV site

Expression

Mutation

D421A, E432K, F433W, F453W

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2(3)-C911

Plasmid

#202452

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2(3)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2(3)

Plasmid

#202451

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgBIRC6-1

Plasmid

#202437

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgHRI

Plasmid

#202443

Purpose

Expression of a guide RNA targeting HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI gRNA (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2

Plasmid

#202449

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2-C911

Plasmid

#202450

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-5

Plasmid

#202448

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-4

Plasmid

#202438

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-5

Plasmid

#202439

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2_1-496-siR

Plasmid

#196515

Purpose

Expression of Myc-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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