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pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

Plasmid

#178999

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

Plasmid

#178998

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

Plasmid

#178997

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

Plasmid

#178994

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

Plasmid

#178993

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

Plasmid

#178992

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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B7RR92

Plasmid

#163246

Purpose

Inducible expression of UniProt identifier B7RR92

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B7RR92

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A9QH69

Plasmid

#163216

Purpose

Inducible expression of UniProt identifier A9QH69

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A9QH69

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A4YVE0

Plasmid

#163217

Purpose

Inducible expression of UniProt identifier A4YVE0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A4YVE0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A087D1R1

Plasmid

#163223

Purpose

Inducible expression of UniProt identifier A0A087D1R1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A087D1R1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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B1HZY7

Plasmid

#163229

Purpose

Inducible expression of UniProt identifier B1HZY7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B1HZY7

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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C4VMW0

Plasmid

#163249

Purpose

Inducible expression of UniProt identifier C4VMW0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C4VMW0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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C0XIJ3

Plasmid

#163227

Purpose

Inducible expression of UniProt identifier C0XIJ3

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C0XIJ3

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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F4G5A4

Plasmid

#163219

Purpose

Inducible expression of UniProt identifier F4G5A4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F4G5A4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C2K1F0

Plasmid

#163272

Purpose

Inducible expression of UniProt identifier C2K1F0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C2K1F0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCD30

Plasmid

#173033

Purpose

pQE30 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

S. flexneri 2a wzzBSF gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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