We narrowed to 12,483 results for: BASE

1. pGEL855

   Plasmid

   #214255

   Purpose

   Expression Td-CBEmax in rice cells

   Depositor

   Yong Zhang

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPO3

   Plasmid

   #209977

   Purpose

   Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (pAMBeta1)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-AAVS1-Ef1a-Blast

   Plasmid

   #227267

   Purpose

   Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xNFAT-ZsG-mCD8

   Plasmid

   #162747

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mAzurite-16xRhoBAST

   Plasmid

   #196347

   Purpose

   Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   mAzurite-16xRhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTcI39-switch

   Plasmid

   #195557

   Purpose

   Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mRFP1
   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21d-myc-BirA

   Plasmid

   #109424

   Purpose

   Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   BirA (birA )
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_078

   Plasmid

   #158582

   Purpose

   Lentiviral expression of BE4max and customizable gRNA

   Depositor

   David Root, John Doench

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRIME-TET-GFP-recipient

   Plasmid

   #11655

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

    Plasmid

    #209296

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT128c

    Plasmid

    #138512

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-13Myc-His3MX6

    Plasmid

    #41602

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBDC

    Plasmid

    #135188

    Purpose

    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    CmR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_256

    Plasmid

    #158581

    Purpose

    Lentiviral expression of BE3.9max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT279

    Plasmid

    #122609

    Purpose

    express FERNY-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-LIC-N8C_Fab4a

    Plasmid

    #203690

    Purpose

    Expresses N8C_Fab4a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid

    #165424

    Purpose

    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Os Ubiquitin promoter :: Cas9 :: NosTerminator

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSyn-EGFP

    Plasmid

    #114213

    Purpose

    AAV vector (control) that targets expression of EGFP to neurons

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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