We narrowed to 5,861 results for: SPI

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LOC5570884 Cardioacceleratory peptide receptor

Plasmid

#115373

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570884 Cardioacceleratory peptide receptor (LOC5570884 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5570885 Cardioacceleratory peptide receptor

Plasmid

#115375

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570885 Cardioacceleratory peptide receptor (LOC5570885 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5574680 Atrial natriuretic peptide receptor

Plasmid

#115374

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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FH95pUP95S73DGW (F3)

Plasmid

#74040

Purpose

lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

Depositor

Robert Malenka, Bernardo Sabatini, Weifeng Xu

Article

Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

S73D

Promoter

H1

Availability

Academic Institutions and Nonprofits only
pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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phMYT1L-N174

Plasmid

#66809

Purpose

2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-tdTomato

Plasmid

#83029

Purpose

expresses tdTomato fluorescent protein under the CAG promoter

Depositor

Angelique Bordey

Article

Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

Insert

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pTK88_GFP-Anillin

Plasmid

#46354

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Anillin (ANLN Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

a1854 is changed to c to disrupt XhoI site withou…

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ230 (LbCpf1)

Plasmid

#86210

Purpose

LbCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV RT∆RNase H

Plasmid

#213749

Purpose

For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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LbCas12a-niCPE

Plasmid

#213747

Purpose

For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-nLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A in LbCas12a

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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