We narrowed to 19,393 results for: IRE
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AAVS1_CAG_OsTIR1_F74G_S210A
Plasmid#242189PurposeAID 2.0's higher degradation efficiency came with target-specific basal degradation and slower recovery rates. To address these limitations, we employed base-editing-mediated mutagenesis followed by sDepositorInsertCAG_OsTIR1_F74G_S210A
ExpressionMammalianMutationS210APromoterCAGAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Puro-Arpp19-short
Plasmid#248798PurposeOverexpression of ARPP19 shortDepositorAvailable SinceDec. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Puro-Arpp19-long
Plasmid#248799PurposeOverexpression of ARPP19 longDepositorAvailable SinceDec. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-hnRNPC
Plasmid#245722PurposeExpress Flag-HA-hnRNPC in mammalian cellsDepositorAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-GFP pLVX CMVpuro
Plasmid#246912PurposeLentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cellsDepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
hPSD-95_PDZ3
Plasmid#245905PurposeBacterial expression of PSD-95 PDZ3 domain (302-402)DepositorAvailable SinceOct. 23, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
hPSD-95_PDZ2
Plasmid#245904PurposeBacterial expression of PSD-95 PDZ2 domain (155-249)DepositorAvailable SinceOct. 23, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-SUMO-SHP2-N-SH2(K55R)-Avi
Plasmid#245186PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationK55RAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-N-SH2(T52S)-Avi
Plasmid#245185PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationT52SAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-N-SH2(L43F)-Avi
Plasmid#245184PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationL43FAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-N-SH2(T42A)-Avi
Plasmid#245183PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationT42AAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-N-SH2(WT)-Avi
Plasmid#245182PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationwild-typeAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-C-SH2(R138Q)-Avi
Plasmid#245189PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 C-SH2
TagsAvi and His-SUMOExpressionBacterialMutationR138QAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSmBtA3-3AF
Plasmid#245785PurposeExpression of mutated bovine arrestin-3 (I385A, V386A, F387A) tagged with SmBiT and Flag in mammalian cellsDepositorInsertarrestin-3 (ARR3 Bovine)
TagsFlag and SmBiTExpressionMammalianMutationI385A, V386A, F387APromoterCMVAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDS1
Plasmid#239460PurposeFor simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusionDepositorTypeEmpty backboneTagsVENUS:3xHA:2A:mTurqN7ExpressionBacterial and PlantAvailable SinceOct. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-N-SH2(E76K)-Avi
Plasmid#245187PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 N-SH2
TagsAvi and His-SUMOExpressionBacterialMutationE76KAvailable SinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-C-SH2(WT)-Avi
Plasmid#245188PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 C-SH2
TagsAvi and His-SUMOExpressionBacterialMutationwild-typeAvailable SinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aga2p-TEVcs-LacAnc100-myc_pCTCON2
Plasmid#245298Purposeexpresses LaccID on the yeast surfaceDepositorInsertAga2p-LacAnc100
ExpressionYeastAvailable SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SS-HA-V5-LacAnc100-CD4 TM_pDisplay
Plasmid#245299Purposeexpresses LaccID on the mammalian cell surfaceDepositorInsertLacAnc100-CD4TM
ExpressionYeastAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-SHP2-C-SH2(E139D)-Avi
Plasmid#245190PurposeProduction of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domainDepositorInsertSHP2 C-SH2
TagsAvi and His-SUMOExpressionBacterialMutationE139DAvailable SinceSept. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS
Plasmid#244842PurposeBacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminusDepositorInsertFlex-Cas12a
UseCRISPRTags10xHis-CL7-2xSV40 NLS and 4xSV40 NLSExpressionBacterialMutationG146R/R182V/D535G/S551F/D665N/E795QPromoterT7Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA ROSA 4b PhiC31 attP41
Plasmid#242881PurposeMammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 siteDepositorInsertepegRNA for PhiC31 attP site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA AAV 4a PhiC31 attP41
Plasmid#242882PurposeMammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 siteDepositorInsertepegRNA for PhiC31 attP site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA AAV 3b PhiC31 attP41
Plasmid#242883PurposeMammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 siteDepositorInsertepegRNA for PhiC31 attP site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA ROSA 4b Bxb1 attB38
Plasmid#242879PurposeMammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 siteDepositorInsertepegRNA for Bxb1 attB site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA ROSA 4a Bxb1 attB38
Plasmid#242878PurposeMammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 siteDepositorInsertepegRNA for Bxb1 attB site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6 pegRNA ROSA 4a PhiC31 attP41
Plasmid#242880PurposeMammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 siteDepositorInsertepegRNA for PhiC31 attP site
ExpressionMammalianMutationnonePromoterU6Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-Nsp3(1-111)
Plasmid#242947PurposeGFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)DepositorAvailable SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-APOX-KDEL
Plasmid#242339PurposeExpresses human APOX in ER/GolgiDepositorInsertAPOX-FLAG-KDEL (APEX2 Human)
TagsFLAG and KDELExpressionMammalianMutationF41V, S69I, Y235F, H239YPromoterCMVAvailable SinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-APOX
Plasmid#242344PurposeExpresses human APOX in cytosolDepositorInsertFLAG-APOX (APEX2 Human)
TagsFLAGExpressionMammalianMutationF41V, S69I, Y235F, H239YPromoterCMVAvailable SinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDS2
Plasmid#239459PurposeSimultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusionDepositorTypeEmpty backboneTags3xHA:VENUS:2A:mTurqN7ExpressionBacterial and PlantAvailable SinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-N2-BE4-puro
Plasmid#226583PurposeExpress N2-BE4 base editor in mammalian cellsDepositorInsertN2-BE4
UseCRISPRExpressionMammalianAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30-LacAnc100
Plasmid#234653PurposeExpresses LacAnc100 variant in yeastDepositorInsertLacAnc100
TagsHRV 3C protease site-GSG linker-8xHis tagExpressionYeastPromoterGal1pAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNSE-NF186WT-HA
Plasmid#239781PurposeExpresses WT rat NF186 and with a C-terminal HA tag in mammalian cells.DepositorInsertNfasc (Nfasc Rat)
TagsHA-tagExpressionMammalianPromoterhNSE (obtained from Addgene plasmid #11606)Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPMEL-Nluc-hbb
Plasmid#239862PurposeTemplate for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.DepositorInsertSecreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
UseLuciferasePromoterT7 (included as part of insert)Available SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10-BioID
Plasmid#145137PurposeExpresses EGFP-MYO10-BioID construct in mammalian cellsDepositorInsertMYO10-BioID (MYO10 Human)
ExpressionMammalianAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-GFP-SV40pA-HAR
Plasmid#225357PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.DepositorInsertT2A-GFP-SV40pA
UseCRISPRAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-HYG-SV40pA-HAR
Plasmid#225358PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.DepositorInsertT2A-HYG-SV40pA
UseCRISPRAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-NEO-SV40pA-HAR
Plasmid#225359PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.DepositorInsertT2A-NEO-SV40pA
UseCRISPRAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_LIG4_Exon3
Plasmid#225363PurposepX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.DepositorAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVeBtA2d7
Plasmid#238989PurposeMammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusionDepositorInsertarrestin-2 long (ARRB2 Bovine)
TagsVenusExpressionMammalianMutationdeleted residues 174, 176, 177, 181-184PromoterCMVAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVEA3-delta7
Plasmid#234802PurposeMammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)DepositorInsertArrestin-3 (ARR3 Bovine)
TagsVenusExpressionMammalianMutationdeleted residues 176, 177, 178, 182-185Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_Z11 gpN:SpyTag
Plasmid#225196PurposeUsed for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS∆tum
Plasmid#225198PurposeUsed to create a markerless deletion of the E. coli HS tum geneDepositorInserttum homology arms
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_lexA(Ind-)
Plasmid#225199PurposeUsed to create a non-inducible lexA by introducing a G85D mutation in E. coli HSDepositorInsertlexA homology arms
ExpressionBacterialMutationlexA G85DAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag
Plasmid#225193PurposeUsed for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x3HA
Plasmid#233189PurposeRetroviral expression of tagged mNgn2x3HADepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x3FLAG
Plasmid#233190PurposeRetroviral expression of tagged mNgn2x3FLAGDepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x1HA
Plasmid#233191PurposeRetroviral expression of tagged mNgn2x1HADepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only
