Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,007 results for: actin

Clear keywords
Showing: 4061 - 4080 of 6007 results

  1. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

    Enlarge

  3. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

    Enlarge

  4. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Publication
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103803-plasmid-map-sequence-id-199226

    Enlarge

  5. pCIBN-EYFP-hTRF1

    Plasmid
    #103802
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Publication
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIBN-EYFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103802-plasmid-map-sequence-id-199212

    Enlarge

  6. pLexM-HsPQLC1(16-271)-TEV-GFP-His8

    Plasmid
    #250419
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-15), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-15 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250419-plasmid-map-sequence-id-499018

    Enlarge

  7. pLexM-HsPQLC1(20-271)-TEV-GFP-His8

    Plasmid
    #250420
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-19), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-19 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250420-plasmid-map-sequence-id-499015

    Enlarge

  8. pLexM-HsPQLC1(24-271)-TEV-GFP-His8

    Plasmid
    #250421
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-23), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-23 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250421-plasmid-map-sequence-id-498996

    Enlarge

  10. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222015-plasmid-map-sequence-id-457427

    Enlarge

  11. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Publication
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

    Enlarge

  12. Cav2.2 BBS exon37a pCAGGS

    Plasmid
    #206086
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a
    Depositor
    Annette Dolphin
    Publication
    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    double Bungarotoxin Binding Site motif (internal …
    Expression
    Mammalian
    Promoter
    CMV/B-actin
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206086-plasmid-map-sequence-id-413048

    Enlarge

  16. pAAV hGRN4

    Plasmid
    #213684
    Purpose
    Expresses human granulin-4 with an N-terminal twin-Strep-FLAG tag
    Depositor
    Thomas Kukar
    Publication
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human granulin-4 (hGRN4) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and FLAG tag (after signal peptide…
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213684-plasmid-map-sequence-id-455752

    Enlarge

  18. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

    Enlarge

Showing: 4061 - 4080 of 6007 results