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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 4061 - 4080 of 14582 results

  1. pHS_Nova

    Plasmid
    #239831
    Purpose
    NovaIscB mammalian expression
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NovaIscB
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    CMV
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239831-plasmid-map-sequence-id-479113

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  5. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  11. pcDNA3.1-CHRFAM7A-mCherry

    Plasmid
    #62635
    Purpose
    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop
    Depositor
    Henry Lester
    Publication
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Expression
    Mammalian
    Mutation
    mCherry inserted into M3-M4 loop
    Promoter
    CMV
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  15. ePE6d

    Plasmid
    #229029
    Purpose
    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
    Depositor
    Li Juan
    Publication
    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10
    Insert
    Cas9
    Use
    CRISPR
    Available since
    Feb. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229029-plasmid-map-sequence-id-462729

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  16. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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  17. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Publication
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122853-plasmid-map-sequence-id-238877

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  19. pBiFC-PBZ-4A-VC

    Plasmid
    #110649
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Publication
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Tags
    HA and VC
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110649-plasmid-map-sequence-id-217742

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Showing: 4061 - 4080 of 14582 results