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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4061 - 4080 of 6962 results

  1. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196053-plasmid-map-sequence-id-387469

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  2. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  3. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  5. BFP Homology Donor

    Plasmid
    #149372
    Purpose
    Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CDDR BFP Homology Donor
    Expression
    Mammalian
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    149372-plasmid-map-sequence-id-313974

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  8. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Publication
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113934-plasmid-map-sequence-id-225307

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  9. LifeAct-mGarnet

    Plasmid
    #104307
    Purpose
    codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells
    Depositor
    Ulrich Nienhaus
    Publication
    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LifeAct-mGarnet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104307-plasmid-map-sequence-id-225629

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  11. EphA2-MT

    Plasmid
    #108851
    Purpose
    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker
    Depositor
    Kalina Hristova
    Publication
    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EPHA2 (EPHA2 Human)
    Tags
    Labeled with mTurquoise on the C-Terminus via a 1…
    Expression
    Mammalian
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    108851-plasmid-map-sequence-id-208238

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  15. FL-mCherry

    Plasmid
    #78862
    Purpose
    DULIP negative control (prey) to exclude unspecific background binding
    Depositor
    Erich Wanker
    Publication
    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    Expression vector
    Tags
    Firefly luciferase, V5-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78862-plasmid-map-sequence-id-253041

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  16. Rlucrarr3-Flash4

    Plasmid
    #74132
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  17. Rlucrarr3-Flash3

    Plasmid
    #74131
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  18. pcDNA-LBR-mRFP1-polyA

    Plasmid
    #59749
    Purpose
    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LBR-mRFP1
    Tags
    mRFP1
    Expression
    Mammalian
    Mutation
    aa 1-249 of LBR
    Promoter
    CMV and T7
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59749-plasmid-map-sequence-id-94053

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Showing: 4061 - 4080 of 6962 results