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pK RagA-RagC(L91P)

Plasmid

#99707

Purpose

Bacterial expression of RagA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(K84T)

Plasmid

#99708

Purpose

Bacterial expression of RagA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

K84T

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(R37P)-RagC

Plasmid

#99646

Purpose

Bacterial expression of RagA(R37P)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

R37P

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(K84T))

Plasmid

#99648

Purpose

Bacterial expression of RagA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

K84T

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA(T21N)-RagC

Plasmid

#99650

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

T21N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(S75N)

Plasmid

#99699

Purpose

Bacterial expression of RagA-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

S75N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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hFLN29G37-end/pCMV-14-FLAG

Plasmid

#73708

Purpose

Express human TRAFD1 (FLN29) from G37 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 36 aa from full lenght TRAFD1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 Q211-end/pCMV-14-FLAG

Plasmid

#73712

Purpose

Express human TRAFD1 (FLN29) from Q211 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 210 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 L175-end/pCMV-14-FLAG

Plasmid

#73711

Purpose

Express human TRAFD1 (FLN29) from L175 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 174 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 G117-end/pCMV-14-FLAG

Plasmid

#73710

Purpose

Express human TRAFD1 (FLN29) from G117 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 116 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 C60-end/pCMV-14-FLAG

Plasmid

#73709

Purpose

Express human TRAFD1 (FLN29) fromC60 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 59 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 DM

Plasmid

#61931

Purpose

Expression of hUCH37 (ISF1) M269E/N291D in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) M269E/N291D (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Changed methionine 269 to glutamate and asparagin…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 (ISF1)(M148A/F149A)

Plasmid

#61933

Purpose

Expression of hUCH37 (M148A/F149A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Methionine 148 and Phenylalanine 149 were mutated…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
PMKO-sh_lnc-BATE1

Plasmid

#64866

Purpose

Expresses shRNA targeting lnc-BATE1

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

shRNA targeting lnc-BATE1

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only