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pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Publication

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Publication

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCLX-UBI-GFP

Plasmid

#27245

Depositor

Patrick Salmon

Publication

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

GFP

Use

Lentiviral

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHiUGE HA-epitope donor ORF1

Plasmid

#200372

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pP2-P1(S')

Plasmid

#196334

Purpose

Plasmid expressing chimeric P2-P1(S') tail fiber

Depositor

Baojun Wang

Publication

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-P1(S') and chaperone protein U'

Expression

Bacterial

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFloxin-IRES-eGFP

Plasmid

#24557

Depositor

Jeremy Reiter

Publication

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

eGFP

Use

Cre/Lox and Mouse Targeting

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET24b-SUMO-BG2.4

Plasmid

#199438

Purpose

expresses BAmGreen2.4 in bacterial cells with a SUMO tag

Depositor

Andrew Woolley

Publication

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen2.4

Tags

his tag and sumo

Expression

Bacterial

Promoter

T7

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHdCas9

Plasmid

#137080

Purpose

Expression of Himar-dCas9 in bacteria

Depositor

Harris Wang

Publication

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Expression

Bacterial

Promoter

pLtetO-1

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBTK229

Plasmid

#110591

Purpose

BTK Type 3 coding sequence for golden gate assembly and homologous recombination

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Kanamycin resistance

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2615

Plasmid

#91078

Purpose

Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BsaI ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pP2-phiV10

Plasmid

#196333

Purpose

Plasmid expressing chimeric P2-phiV10 tail fiber

Depositor

Baojun Wang

Publication

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-phiV10

Expression

Bacterial

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-Ran(Q69L)

Plasmid

#171563

Purpose

Encodes human Ran protein

Depositor

Yan Zeng

Insert

Ran (RAN Human)

Expression

Bacterial

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Publication

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Publication

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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