We narrowed to 5,835 results for: pcas

1. pGWB-nLUC

   Plasmid

   #174050

   Purpose

   Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   N-terminal luciferase

   Expression

   Plant

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tad-CBE2.4

   Plasmid

   #235055

   Purpose

   Expresses Tad-CBE2.4 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE2.4-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-mCherry-LacZshRNA

   Plasmid

   #73978

   Purpose

   LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the LacZ gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tad-CBE3.6

   Plasmid

   #235058

   Purpose

   Expresses Tad-CBE3.6 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE3.6-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRANS_250d

   Plasmid

   #91124

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   PvUbi2

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

   Plasmid

   #71878

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC-delta (Otc Rat)
   

   Mutation

   Deleted amino acid 30-114 from full length OTC

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ePE6d

   Plasmid

   #229029

   Purpose

   Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

   Depositor

   Li Juan

   Article

   Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIVT-Cas9-DEN2

    Plasmid

    #238017

    Purpose

    for DNA-free gene knockout editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    SpCas9
    

    Expression

    Plant

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEP101E

    Plasmid

    #97387

    Purpose

    binary construct of cytosolic sfGFP1-10 for plant

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Expression

    Plant

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBI-CRY2PHR-VPR

    Plasmid

    #192606

    Purpose

    Plasmid for constitutive expression of CRY2PHR-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    CRY2PHR-VPR
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA2058_pX330A-G2P

    Plasmid

    #124202

    Purpose

    Expression of SpCas9 and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFPL-Gh

    Plasmid

    #61648

    Purpose

    Gateway vector for generating green fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced green fluorescent protein
    

    Expression

    Plant

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Promoter

    U6-96Ab and hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXY027

    Plasmid

    #98915

    Purpose

    Express FtsZ tagged with GFP in E coli

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    FtsZ
    

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTalpha1-Dre

    Plasmid

    #133925

    Purpose

    Neuron-specific expression of Dre

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    HA-Dre
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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