We narrowed to 18,932 results for: otos

1. pMRX-IP-3xFLAG-NCOA4 (dM)

   Plasmid

   #192810

   Purpose

   Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of the Middle domain

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-3xFLAG-NCOA4 (dIDR1)

   Plasmid

   #192809

   Purpose

   Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of the IDR1

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRX-IP-3xFLAG-NCOA4 (dC)

   Plasmid

   #192812

   Purpose

   Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of the C-terminal domain

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-3xFLAG-NCOA4 (I56E)

   Plasmid

   #192813

   Purpose

   Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I56E mutation in the N-terminal domain

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-3xFLAG-NCOA4 (L63R)

   Plasmid

   #192814

   Purpose

   Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L63R mutation in the N-terminal domain

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWZL-Blast-Flag-HA-hTERT

   Plasmid

   #22396

   Depositor

   William Hahn

   Article

   Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

   Insert

   TERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-Mito-mRFP

   Plasmid

   #169001

   Purpose

   Express Mito (Su9)-mRFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
   

   Use

   Unspecified

   Tags

   mRFP

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-DIO-mTurquoise2

   Plasmid

   #104059

   Purpose

   AAV expression of Cre-dependent reporter mTurquoise2

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-X(5010)B1

   Plasmid

   #168019

   Purpose

   Expresses X(5010)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(5010)B1-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWP78-PVCpnf_pvc13-truncated

    Plasmid

    #198286

    Purpose

    Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-truncated
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-CV-SNAP25

    Plasmid

    #168031

    Purpose

    Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-SNAP25(A141-G206)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIbsr-EKARrEV-NLS

    Plasmid

    #173854

    Purpose

    A lentiviral vector for EKARrEV-NLS

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-T2A-moxMaple3

    Plasmid

    #120875

    Purpose

    Mammalian expression of mCherry and moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Inserts

    mCherry
    moxMaple3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IZ-ANXA7-mRuby3

    Plasmid

    #184893

    Purpose

    Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA7 (ANXA7 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCS-H2B-EGFP

    Plasmid

    #53744

    Purpose

    Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    June 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Fus-BcLOV-mCh-SOS

    Plasmid

    #208275

    Purpose

    Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-X(4130)B1

    Plasmid

    #168018

    Purpose

    Expresses X(4130)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(4130)B1-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti_MS2_P65_HSF1_2A_puro

    Plasmid

    #118699

    Purpose

    To express MS2-P65-HSF1

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMRXIP-GFP-YKT6

    Plasmid

    #116946

    Purpose

    Expressing GFP-YKT6 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    Synaptobrevin homolog YKT6 (YKT6 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

    Plasmid

    #192636

    Purpose

    Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Teto BRN3A

    Plasmid

    #62221

    Purpose

    human BRN3A cDNA

    Depositor

    Kristin Baldwin

    Article

    Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

    Insert

    BRN3A (POU4F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted a.a. 142-181

    Promoter

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAWP78-PVCpnf_pvc12-HiBiT

    Plasmid

    #198308

    Purpose

    Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc12-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209658

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 CDK7 HA (P#633)

    Plasmid

    #14647

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge