We narrowed to 7,812 results for: sms

1. sgCTRL-hygro

   Plasmid

   #199639

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV10-Flag-Ovtm

   Plasmid

   #51888

   Purpose

   expression vectore in mammalian cells

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   mFbxl3 (Fbxl3 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   ovtm (I364T)

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-FullLength-TET1-CXXC/LLAA

   Plasmid

   #124399

   Purpose

   Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CXXC zinc finger deletion (aa 585-624) and L897A/…

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CDK16_HUMAN_D0

   Plasmid

   #79696

   Purpose

   This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CDK16 (CDK16 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pJWK_VD_02

   Plasmid

   #158103

   Purpose

   5'UTR stem-loop luciferase reporter: -30kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop -30kcal/mol
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBullet-pm-c

   Plasmid

   #53078

   Purpose

   destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-M-C4

   Plasmid

   #73427

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

   Plasmid

   #91882

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pαH-S-HRV3C/D614G

   Plasmid

   #164568

   Purpose

   SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-HRV3C-D614G variant)
   

   Tags

   2X Strep-Tag II and 8X His tag

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CHK2_HUMAN_D0

    Plasmid

    #79715

    Purpose

    This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CHK2 (CHEK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

    Plasmid

    #163138

    Purpose

    Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86023

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain: Changed threonine 230 to…

    Promoter

    CMV IE

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86024

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain: Changed aspartic acid 63…

    Promoter

    CMV IE

    Available Since

    April 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBullet-cg-c

    Plasmid

    #53070

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DAPK1_HUMAN_D0

    Plasmid

    #79728

    Purpose

    This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DAPK1 (DAPK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pdCas9-M-3F2

    Plasmid

    #73428

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBullet-vac-c

    Plasmid

    #53076

    Purpose

    destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBullet-er-c

    Plasmid

    #53068

    Purpose

    destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86017

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Changed threonine 230 to alanine

    Promoter

    CMV IE

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86018

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Changed aspartic acid 637 to glycine

    Promoter

    CMV IE

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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