We narrowed to 19,702 results for: ACE

1. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

   Plasmid

   #160045

   Purpose

   For expressing the active auxin pump PIN2

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   PIN2
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   pEF1s

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPomyces-1

   Plasmid

   #61736

   Purpose

   Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   tracrRNA
   crRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

   Plasmid

   #60624

   Purpose

   Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   WT mouse MyoD (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG.PKCzeta.T410A

   Plasmid

   #10801

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Activation loop inactive: T410A (Thr 410 to Ala)

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

6. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mNeonGreen-LEHD-NanoLuc

   Plasmid

   #98289

   Purpose

   caspase-9 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-2xCL-YFP-Vpr

   Plasmid

   #105215

   Purpose

   Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

   Depositor

   Gregory Melikyan

   Article

   Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

   Insert

   Vpr
   

   Use

   Tags

   PC2x (SQNY/IRKVL), eYFP, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVA

    Plasmid

    #121149

    Purpose

    This plamid was used for improving the production of DMAPP (IPP) in E.coli

    Depositor

    Minjuan Xu

    Article

    He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

    Insert

    MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLD2 translation factors

    Plasmid

    #117761

    Purpose

    BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 14-21
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNSA-ARS-CRISPR-BlastR

    Plasmid

    #101009

    Purpose

    Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-YEE-BE3

    Plasmid

    #85177

    Purpose

    Expresses YEE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2_Renilla_luc-60A

    Plasmid

    #200397

    Purpose

    A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-Caspase-3(D3A,V266E)

    Plasmid

    #90091

    Purpose

    Expresses caspase D3A and V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A V266E

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRBC-sfGFP-134/150TAG

    Plasmid

    #174077

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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