We narrowed to 6,488 results for: sas

1. pHD-FlpTag-DsRed-HDR-ph1

   Plasmid

   #231901

   Purpose

   dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

   Depositor

   Alexander Borst

   Article

   Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

   Insert

   Frt-Frt14-SD-GFP-SA-Frt-Frt14
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHD-FlpTag-DsRed-HDR-ph0

   Plasmid

   #231900

   Purpose

   dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

   Depositor

   Alexander Borst

   Article

   Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

   Insert

   Frt-Frt14-SD-GFP-SA-Frt-Frt14
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS_KS_attB2_SA(2)_T2AGAL80

   Plasmid

   #125219

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL80
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHJ33

   Plasmid

   #48362

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLBCX-Large-T-antigenK1 mutant

   Plasmid

   #189978

   Purpose

   Retroviral vector used to immortalize human cells

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   Large-T-antigenK1 mutant
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-sgTrp53-sgAtrx-EFS-Cre

   Plasmid

   #189977

   Purpose

   Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   sgTrp53, sgAtrx, Cre recombinase
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-MerCreMer-hygro

   Plasmid

   #188982

   Purpose

   Retroviral expression of tamoxifen-inducible Cre recombinase

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   Tamoxifen-inducible Cre recombinase
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-H2B-EGFP HR donor vector

    Plasmid

    #137925

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R26-H2B-mCherry HR donor vector

    Plasmid

    #137928

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-mCherry-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHJ17

    Plasmid

    #48356

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHJ22

    Plasmid

    #48358

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R26-EGFP MMEJ donor vector

    Plasmid

    #137926

    Purpose

    R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with micro-homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV_A2S-8

    Plasmid

    #188654

    Purpose

    AAV vector expressing SaAID-2S and gRNA

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S--nSaCas9-UGI
    Sa-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    Scp1 and U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHJ18

    Plasmid

    #48357

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHJ41

    Plasmid

    #48360

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge