We narrowed to 9,659 results for: BLI

1. 3'dmDR-CMV-CasRx-bGHpolyA

   Plasmid

   #229556

   Purpose

   Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

   Depositor

   Omar Akbari

   Insert

   CasRx
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

   Plasmid

   #221448

   Purpose

   Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3

   Depositor

   Omar Akbari

   Inserts

   wtCas9-NG
   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLaG2-T7RNAPC

   Plasmid

   #216337

   Purpose

   Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP

   Depositor

   Mark Styczynski

   Insert

   LaG2-T7RNAPC fusion
   

   Expression

   Bacterial

   Mutation

   g2658a, g3915a

   Available Since

   Jan. 17, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBS-ACTB-C200-T7

   Plasmid

   #229850

   Purpose

   Donor vector for the ACTB site containing T7 sequence for detection of HR products.

   Depositor

   Natsuko Chiba

   Insert

   Partial sequence of ACTB gene
   

   Use

   Hr donor vector for human actb gene.

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG-scr

   Plasmid

   #229847

   Purpose

   Expresses wild-type Cas9 and scrambled gRNA.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA with scrambled sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDual_dsCas9_Venus_hs_NC_chr1

   Plasmid

   #214682

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual_dsCas9_Venus_hs_NC_chr2

   Plasmid

   #214683

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr2
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDual_dsCas9_Venus_hs_TP73

   Plasmid

   #214685

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73

   Depositor

   Kevin Janes

   Insert

   dgRNA_TP73 (TP73 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDual_dsCas9_BFP_hs_NC_chr1

   Plasmid

   #214686

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDual_dsCas9_BFP_hs_TP73

    Plasmid

    #214687

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-TRE-miRFP703_PGK-Puro

    Plasmid

    #216667

    Purpose

    Expresses tTa-inducible miRFP703, with puromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    miRFP703
    PuroR
    

    Use

    Lentiviral

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti puro HA-TUBA1B

    Plasmid

    #227587

    Purpose

    Lentiviral plasmid expressing human TUBA1B protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    TUBA1B (TUBA1B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-phSyn-flex-SypHaloTag

    Plasmid

    #172280

    Purpose

    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

    Depositor

    Danny Winder

    Insert

    synaptophysin-HaloTag
    

    Use

    AAV and Cre/Lox

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    rat synapsin

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MDH2_EC

    Plasmid

    #224146

    Purpose

    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.

    Depositor

    Katherine Walstrom

    Insert

    MDH-2 (mdh-2 Nematode)
    

    Tags

    His tag, Xpress tag, enterokinase cleaveage site

    Expression

    Bacterial

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIND4-GFP-MmXYRS

    Plasmid

    #222745

    Purpose

    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker

    Depositor

    Steven Boxer

    Inserts

    sfGFP
    MmXYRS
    Mm Pyl tRNA
    

    Tags

    6xHis

    Mutation

    Contains the following substitutions: V346A, W348…

    Promoter

    rrnB

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #223843

    Purpose

    Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons

    Depositor

    Bosiljka Tasic

    Insert

    iCre(R297T)
    

    Use

    AAV

    Mutation

    Argenine in aa297 was replaced with Threonine

    Promoter

    minBG

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. H2B-Electra2

    Plasmid

    #218929

    Purpose

    Electra2 fused to human H2B protein for nuclear localization

    Depositor

    Amro Hamdoun

    Insert

    H2B-Electra2 (H2B Synthetic, Human, Entacmaea quadricolor)
    

    Use

    Synthetic Biology

    Tags

    Electra2 and H2B

    Expression

    Mammalian

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LvPolyUb

    Plasmid

    #218982

    Purpose

    L.variegatus polyubiquitin-C promoter

    Depositor

    Amro Hamdoun

    Insert

    L.variegatus polyubiquitinC promoter
    

    Use

    Sea urchin

    Promoter

    L.variegatus polyubiquitinC promoter

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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