We narrowed to 11,601 results for: 112

1. pETM30-ARC105

   Plasmid

   #133426

   Purpose

   Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   PCQAP (MED15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains amino acids 5-78 fused to C-terminal of …

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-FOXQ1

   Plasmid

   #101628

   Purpose

   Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   FOXQ1 (FOXQ1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DDR1 gRNA (BRDN0001162484)

   Plasmid

   #78011

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DDR1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DDR1 (DDR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDR1 gRNA (BRDN0001162509)

   Plasmid

   #78012

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DDR1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DDR1 (DDR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-KIF5A tail only

   Plasmid

   #166956

   Purpose

   Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   KIF5A (820-1027aa)

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_MFSD12

   Plasmid

   #131940

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

   Plasmid

   #153538

   Purpose

   AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   EF1α1.1

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN879

   Plasmid

   #190693

   Purpose

   Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

   Depositor

   Shinsuke Niwa

   Article

   Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

   Insert

   mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-IDH1-R132L

   Plasmid

   #116411

   Purpose

   Lentiviral expression of IDH1 R132L

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R132L

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. B52 + CHEK2 sgSTOP

    Plasmid

    #100712

    Purpose

    B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-AMIGO3-COMP5AP-AviTag-9xHis

    Plasmid

    #157369

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    AMIGO3 (AMIGO3 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti/TO/EYFP-P2A-IDH1(HA)

    Plasmid

    #122482

    Purpose

    A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

    Depositor

    Eric Huang

    Article

    Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

    Insert

    isocitrate dehydrogenase 1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    HA and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ankyrin-G scFv [N106/20]

    Plasmid

    #190525

    Purpose

    Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-Ankyrin-G [N106/36]

    Plasmid

    #190297

    Purpose

    Mammalian Expression Plasmid of anti-Ankyrin-G (Human). Derived from hybridoma N106/36.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Ankyrin-G (Homo sapiens) recombinant Mouse monoclonal antibody (ANK3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IDH1-P33S

    Plasmid

    #116409

    Purpose

    Lentiviral expression of IDH1 P33S

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P33S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-AVITEV- hNFATc1/aA

    Plasmid

    #74057

    Purpose

    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform alpha-A (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    mutation G1157A [R235Q] and the silent mutation C…

    Promoter

    pMSCV-LTRs

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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