We narrowed to 3,581 results for: Gla

1. ORF18 (Y85A)-3xFLAG pCDNA4

   Plasmid

   #129786

   Purpose

   Expresses KSHV ORF18 (Y85A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Tyrosine 85 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ORF18(R74A_K75A)-3xFLAG pCDNA4

   Plasmid

   #129811

   Purpose

   Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Arginine 74 to Alanine and Lysine 75 to A…

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ORF18(W170A_G171A)-3xFLAG pCDNA4

   Plasmid

   #129815

   Purpose

   Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Tryptophan 170 to Alanine and Glycine 171…

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ORF18(R158A)-3xFLAG pCDNA4

   Plasmid

   #129801

   Purpose

   Expresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Arginine 158 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ORF18(G145A)-3xFLAG pCDNA4

   Plasmid

   #129796

   Purpose

   Expresses KSHV ORF18 (G145A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 145 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ORF18(N146A)-3xFLAG pCDNA4

   Plasmid

   #129798

   Purpose

   Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Asparagine 146 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFloxin-Ofd1-Myc-KDD359-361FSY

   Plasmid

   #24578

   Depositor

   Jeremy Reiter

   Article

   Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Myc

   Expression

   Mutation

   Lysine-Aspartate-Aspartate 359-361 changed to Phe…

   Promoter

   Available since

   May 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPT234

   Plasmid

   #127855

   Purpose

   Repressilator 2.0, without degradation tags, integrated triple reporter

   Depositor

   Johan Paulsson

   Article

   Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

   Inserts

   bla
   mSCFP3
   mKate2
   mVenus
   TetR
   LacI
   cI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   First 11aa derived from mCherry as fusion protein

   Promoter

   pAmpR, pL LacO1, pL tetO1, and pR

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX C_K nsp1 CoV2

    Plasmid

    #175516

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX nsp1 R99A CoV2

    Plasmid

    #175515

    Purpose

    For protein expression of R99A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R99A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-UL87-2xStrep

    Plasmid

    #138434

    Purpose

    Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87 (UL87 HCMV (HHV-5))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge