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HL3721
Bacterial Strain#52950PurposeHL716 + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCDDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL4142
Bacterial Strain#37981DepositorBacterial ResistanceChloramphenicolAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3s-ABE7.10
Plasmid#113128Purposeexpressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimizedDepositorInsertABE 7.10
UseTagsHA epitope and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1613
Plasmid#142810PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTADA2A (TADA2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1612
Plasmid#141849PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTADA2A (TADA2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B
Plasmid#67523PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB1677 REPAIR.t3
Plasmid#176320PurposeCMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1923 REPAIR.t1-S
Plasmid#176321PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q/E620G/Q696L, only the deaminase domain (aa …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1676 REPAIR.t1
Plasmid#176319PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Akaby
Bacterial Strain#184224PurposeAkaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.DepositorBacterial ResistanceKanamycinAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMH024
Plasmid#186636PurposeaadA.co between homology arms for pilADepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH036
Plasmid#186637PurposeaadA.co between homology arms for NSIDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH043
Plasmid#186638PurposeaadA.co between homology arms for oscillinDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-mCherry-C1C2
Plasmid#178425PurposeFluorescence reporter fused to EV membrane binding domain for EV imaging and trackingDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSDT-AII
Plasmid#27179DepositorInsertMC1 promoter diphtheria toxin-A fragment
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMODloxZeo3DAmp
Plasmid#27182DepositorInsertlox-EM7p-Zeo-lox cassette flanked by Tn5 outer element
UseIn vitro transposionTagsExpressionMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcS-NP-C1C2
Plasmid#178427PurposeNon peptide (NP) as negative control fused to EV membrane binging domain (C1C2)DepositorInsertNP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcS-p88-C1C2
Plasmid#178424Purposep88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domainDepositorInsertp88
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only
