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pJET1.2-U3

Plasmid

#173156

Purpose

Single guide RNA cassette under U3 promoter

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Insert

sgRNA cassette under U3 promoter

Expression

Plant

Promoter

promoter region of the U3C snRNA gene (Marshallsa…

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS::AtHAP2-V5

Plasmid

#132958

Purpose

Express AtHAP2 full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

AtHAP2 (GCS1, HAP2 Mustard Weed)

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBI101-pGC1(D1)-GUS

Plasmid

#49513

Purpose

Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

Depositor

Julian Schroeder

Article

Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

Insert

Promoter of GC1(D1) (AT1G22690 Mustard Weed)

Use

Plant expression

Tags

beta-glucuronidase

Promoter

GC1 (At1g22690), 1141 bp upstream of the transcri…

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pSW554 - pOp5:SP-mVenus-HDEL

Plasmid

#116006

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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cARA12-mRFP

Plasmid

#181961

Purpose

ARA12 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ARA12 (ARA12 Mustard Weed)

Tags

mRFP

Expression

Plant

Promoter

TBA2

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVL94 - pOp4:VND7

Plasmid

#116016

Purpose

destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR

Expression

Plant

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX2T AtFC1

Plasmid

#136363

Purpose

Bacterial expression of recombinant Arabidopsis thaliana ferrochelatase1

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

Full-length cDNA of Arabidopsis thaliana ferrochelatase1 (FC1 Mustard Weed)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINF AtMKK5

Plasmid

#228396

Purpose

Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

MKK5 (MKK5 Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP50

Plasmid

#196788

Purpose

Contains ACS2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

150 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP51

Plasmid

#196789

Purpose

Contains AOS promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ALLENE OXIDE SYNTHASE (AOS Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

2088 G->C ; 1434 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP55

Plasmid

#196793

Purpose

Contains PDF1.2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT DEFENSIN 1.2 (PDF1.2 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP56

Plasmid

#196794

Purpose

Contains PR1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PATHOGENESIS-RELATED GENE 1 (PR1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP27

Plasmid

#196765

Purpose

Contains CPK5 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CALMODULIN-DOMAIN PROTEIN KINASE 5 (CPK5 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP30

Plasmid

#196768

Purpose

Contains GLR2.7 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

GLUTAMATE RECEPTOR 2.7 (GLR2.7 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP36

Plasmid

#196774

Purpose

Contains DORN1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

DOES NOT RESPOND TO NUCLEOTIDES 1 (LecRK-I.9 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP41

Plasmid

#196779

Purpose

Contains WRKY33 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 33 (WRKY33 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP14

Plasmid

#196752

Purpose

Contains LYM1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

LYSM DOMAIN GPI-ANCHORED PROTEIN 1 PRECURSOR (LYM1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

PIP1;4

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

Plasmid

#172318

Purpose

CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT8163 (AATG_ttLbCas12a-i_TTCG)

Plasmid

#192453

Purpose

intronized ttLbCas12a for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Mutation

temperature-tolerant mutation (D156R)

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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