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Showing: 401 - 420 of 3118 results

  3. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109546-plasmid-map-sequence-id-223868

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  4. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-mCh-KDEL
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  8. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-smFP(myc)-KDEL
    Use
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173021-plasmid-map-sequence-id-339210

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  9. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid
    #177722
    Purpose
    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Use
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177722-plasmid-map-sequence-id-352603

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  11. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Use
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

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  14. mScarletI3-6DG5-MAPPER

    Plasmid
    #222421
    Purpose
    To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    MAPPER
    Use
    Tags
    mScarletI3-6DG5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222421-plasmid-map-sequence-id-439142

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  19. pStr-KDEL_TPV-CXCL9-mCherry-SBP

    Plasmid
    #170677
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    CXCL9 (CXCL9 Synthetic, Human)
    streptavidin-KDEL
    Use
    Tags
    SBP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170677-plasmid-map-sequence-id-335797

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Showing: 401 - 420 of 3118 results