Purpose
As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
Inserts
PrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
Promoter
PrhaBAD rhamnose-inducible promoter from E. coli …
Available Since
May 1, 2017
Availability
Academic Institutions and Nonprofits only