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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 559 results for: mak

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Showing: 401 - 420 of 559 results

  2. pNS3

    Plasmid
    #131770
    Purpose
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites
    Depositor
    Jared Toettcher
    Publication
    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d
    Insert
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    Use
    Integration into δ-sites
    Expression
    Yeast
    Mutation
    E490G to make Cry2 into Cry2Olig
    Promoter
    PADH1
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    131770-plasmid-map-sequence-id-257191

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  3. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid
    #246438
    Purpose
    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmeCas9
    Nme-sgRNA
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 16 to Alanine, deleted amin…
    Promoter
    EF-1 alpha and U6
    Available since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246438-plasmid-map-sequence-id-490930

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  4. pTBL680 CHYRON3 integration construct

    Plasmid
    #126448
    Purpose
    To integrate the CHYRON3 locus at AAVS1 in human cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    Expression
    Mammalian
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    CMV and human U6/3xLacO
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126448-plasmid-map-sequence-id-246061

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  8. Sema3f(S, +)-AP-His

    Plasmid
    #72023
    Purpose
    Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3f (Sema3f Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72023-plasmid-map-sequence-id-131209

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  9. MBC2-null

    Plasmid
    #162749
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes
    Depositor
    Maki Nakayama
    Publication
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162749-plasmid-map-sequence-id-320949

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  10. MBC1-null

    Plasmid
    #162748
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes
    Depositor
    Maki Nakayama
    Publication
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162748-plasmid-map-sequence-id-320948

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  14. pcDNA3-Ro1

    Plasmid
    #24504
    Depositor
    Bruce Conklin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RASSL Ro1 (OPRK1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    mutations in extracellular loop 2, making it a RA…
    Available since
    June 25, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Publication
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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Showing: 401 - 420 of 559 results