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pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]

Plasmid

#107846

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All hydrophobics combined in six clusters in spli…

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]

Plasmid

#107841

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of C-terminal domain in splicing reporter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]

Plasmid

#107840

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of RRM domains in splicing reporter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::zwf_fbp_opcA

Plasmid

#101846

Purpose

Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

sp/st resistance casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::gnd

Plasmid

#101844

Purpose

drives expression of gnd using Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kan casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_EF_syn_BbsI

Plasmid

#89921

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_HI_syn_BbsI

Plasmid

#89924

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST635- BB3_L_AC_hyg_KanOri_pyrG5

Plasmid

#90280

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AC

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_T_34_ani_trpC

Plasmid

#90291

Purpose

BB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

trpC

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_L_12_syn_BsaI

Plasmid

#89914

Purpose

Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_L_34_syn_BsaI

Plasmid

#89916

Purpose

Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_AB_syn_BbsI

Plasmid

#89917

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_BC_syn_BbsI

Plasmid

#89918

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_CD_syn_BbsI

Plasmid

#89919

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_DE_syn_BbsI

Plasmid

#89920

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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