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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Publication

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-hTRAF3 88-C

Plasmid

#44034

Depositor

Shao-Cong Sun

Publication

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 89-568; V274D and S393G

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F2 LUC

Plasmid

#22428

Depositor

William Sessa

Publication

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F2 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Publication

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Publication

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Publication

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Olig2 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRRLsin.PPT.CMV‐GFP‐Eps8 WT

Plasmid

#74922

Purpose

Lentiviral plasmid expressing Eps8 fused to GFP

Depositor

Giorgio Scita

Publication

Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

Insert

Eps8 (Eps8 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
Strain HK022 JQ929581

Bacterial strain

#38204

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Bacterial resistance

Kanamycin

Species

Synthetic

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pMXS-IP Keima-Pex11B

Plasmid

#224558

Purpose

expresses Keima fused to PEX11B in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Publication

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX11B (PEX11B Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V/D38A

Plasmid

#221073

Purpose

Expresses NRAS G12V/D38A in mammalian cells

Depositor

Hidenori Ichijo

Publication

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Insert

NRAS (NRAS Human)

Expression

Mammalian

Mutation

G12V/D38A

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-RAB3A-WT

Plasmid

#206146

Purpose

PGK EGFP-RAB3A-WT

Depositor

Erika Holzbaur

Publication

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

RAB3A (RAB3A Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pVG49

Plasmid

#164707

Purpose

yEVenus under the control of GAL1 promoter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GAL1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+mTurquoise2-Golgi

Plasmid

#128425

Purpose

Contains insert allowing in-vivo visualization of Golgi apparatus

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

mTurquoise2-Golgi

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWT055f

Plasmid

#96865

Purpose

sgRNA-FANCF

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-FANCF

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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