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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 4241 - 4260 of 14067 results

  2. JPF0419d

    Plasmid
    #124020
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124020-plasmid-map-sequence-id-262655

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  3. pOCC120

    Plasmid
    #118892
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mEGFP-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mEGFP, 6xHis,…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118892-plasmid-map-sequence-id-232375

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  4. pEMS1611

    Plasmid
    #29276
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple139 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29276-plasmid-map-sequence-id-13691

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  5. pEMS1612

    Plasmid
    #29277
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple140 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29277-plasmid-map-sequence-id-13692

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  6. pOCC70

    Plasmid
    #118878
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric EGFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-mEGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mEGFP, cleavable with TEV protease; 6xHis, cleava…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118878-plasmid-map-sequence-id-232355

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  9. pOCC305

    Plasmid
    #118898
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118898-plasmid-map-sequence-id-232364

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  11. SND1 (SND1A-c003)

    Plasmid
    #98242
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SND1A (SND1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00567:TUDOR 677-799
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98242-plasmid-map-sequence-id-193420

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  12. p3.2mar-GFP

    Plasmid
    #63694
    Purpose
    3.2 kb marine armor plate enhancer driving GFP reporter
    Depositor
    David Kingsley
    Publication
    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3.2 kb marine stickleback armor plate enhancer
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    hsp70
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63694-plasmid-map-sequence-id-106193

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  16. AAVS1-eTLR

    Plasmid
    #214737
    Purpose
    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-eTLR (PPP1R12C Human, Rabbit, Chicken, Bovine, Synthetic, CMV, Anguilla japonica, influenza)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214737-plasmid-map-sequence-id-425005

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  18. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Publication
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  19. pGL3-Basic-hCYP26A1-FL- luciferase

    Plasmid
    #135566
    Purpose
    Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase
    Depositor
    Catharine Ross
    Publication
    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Full-length promoter of human retinoic acid receptor-beta gene
    Promoter
    hHuman CYP26A1 gene promoter
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135566-plasmid-map-sequence-id-268053

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Showing: 4241 - 4260 of 14067 results