We narrowed to 15,789 results for: LEA

1. TR-Csy4-H29A

   Plasmid

   #80602

   Purpose

   Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-H29A
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   His29 mutated to Ala

   Promoter

   CBA

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

   Plasmid

   #178868

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ-NF-L1-dMb2Cas12a

   Plasmid

   #177683

   Purpose

   Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   FokI-dMb2Cas12a
   

   Use

   CRISPR; Gateway compatible foki-dmb2cas12a entry …

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-PB1-AG(Y63L)-LOV2

   Plasmid

   #178876

   Purpose

   Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen(Y63L)-LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AzamiGreen: Y63L

   Promoter

   CAG

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-104

   Plasmid

   #79842

   Purpose

   Expresses NLS-PpsR2-VP16 under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16
   

   Use

   Tags

   Nuclear localization signal of nuclear cap-bindin…

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG710_lexO-HO_LexA-TF_HIS3MX

   Plasmid

   #154819

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 FLIPE-100u

   Plasmid

   #13543

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

   Plasmid

   #208724

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP443

    Plasmid

    #216361

    Purpose

    xNup98-Nb1t (+1Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup98-Nb1t (+1Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 sgFIP200

    Plasmid

    #175024

    Purpose

    CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    RB1CC1 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNb4OMT-A53M

    Plasmid

    #216232

    Purpose

    Expresses a mutant Norbelladine 4'-O-Methyltransferase

    Depositor

    Andrew Ellington, Simon d'Oelsnitz

    Article

    d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

    Insert

    Nb4OMT-A53M
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    A53M

    Promoter

    P150

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pet15b-Internalization Control

    Plasmid

    #186553

    Purpose

    Negative Control mediating endosomal localization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-EGFP
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJG380

    Plasmid

    #91188

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9+gNt_R2+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Cas12i-Max-T2A-EGFP

    Plasmid

    #188268

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-Max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Cas12i-HiFi-T2A-EGFP

    Plasmid

    #188269

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-HiFi
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+MT-hToca-1-W518K

    Plasmid

    #33031

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    W518K

    Promoter

    simian CMV IE94

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-HA-NLGN4 D396*

    Plasmid

    #59321

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN4 D396* (NLGN4X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D396* nonsense mutation, rest of ORF deleted

    Promoter

    CAG

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T7-RelA(K221R)

    Plasmid

    #23248

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K221R

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only