We narrowed to 14,321 results for: cas9
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Plasmid#118169PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2
Plasmid#118170PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry
Plasmid#170182PurposeMammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptideDepositorInsertmCherry
Tags3XFlag and T2AExpressionMammalianPromoterCBhAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX
Plasmid#163543PurposeMammalian CG-to-GC base editingDepositorInsertPOLD2-APOBEC1-UdgX-nCas9-UdgX
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgPenk
Plasmid#159916PurposeMutagenesis of PenkDepositorInsertPenk (Penk Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pinducer20-dCas9-SunTag-2A-BFP
Plasmid#115779Purposetargeted DNA methylation toolDepositorInsertdCas9-SunTag-2A-BFP
ExpressionMammalianPromoterTREAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S
Plasmid#149638Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, emptyDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR5-NG
Plasmid#120228PurposepCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.46
Plasmid#193284PurposeExpression of CBE-T1.46 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.46
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KNL1-s24a_s60a-dCas9
Plasmid#248568PurposeA CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeresDepositorInsertKNL1-s24a_s60a-dCas9
UseCRISPRTagsHA, 2xNLSExpressionMammalianMutationKNL1 S24A, S60A; Cas9 D10A, H840APromoterCMV, TetO2Available sinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-dCas9-mCherry-CRY2
Plasmid#249579PurposeLentiviral vector for stable expression of dCas9-mCherry-CRY2hiclu fusion protein in mammalian cellsDepositorInsertsCRY2hiclu
Sp dCas9
UseLentiviralTagsmCherryExpressionMammalianMutationCatalytically dead mutant Cas9PromoterCMVAvailable sinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pb-CAGGS-dCas9-VP64-blasto
Plasmid#218556PurposeEncodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistanceDepositorInsertdCas9
UseCRISPR; Piggybac transpositionTags2xSV40NLS-VP64ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…Available sinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgHTT1-7sk-sgCas9
Plasmid#190900PurposeAAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAsDepositorAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-CBP core
Plasmid#179556Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (BB)-2A-GFP gamma-tubulin sg
Plasmid#104437PurposeKnockdown the expression of gamma-tubulin in human cellsDepositorInsertgamma-tubulin sgRNA
TagsThe gamma-tubulin sgRNA is coexpressed with 3xFLA…ExpressionMammalianAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IVT-nCas9(H840A)
Plasmid#207455Purposein vitro transcription of mRNADepositorInsertH840A
ExpressionBacterialAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only