Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 11,256 results for: NSI

Showing: 8521 - 8560 of 11256 results

  1. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pMCh-1540

    Plasmid
    #82433
    Purpose
    Plasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    EF1A
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82433-plasmid-map-sequence-id-156169

    Enlarge

  13. pDONR223 SARS-CoV-2 NSP12

    Plasmid
    #141265
    Purpose
    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141265-plasmid-map-sequence-id-288081

    Enlarge

  22. pDONR207 SARS-CoV-2 NSP3

    Plasmid
    #141257
    Purpose
    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141257-plasmid-map-sequence-id-288044

    Enlarge

  24. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid
    #125712
    Purpose
    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    eOPN3
    Use
    AAV
    Tags
    Rho1D4 and mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 331-429
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    125712-plasmid-map-sequence-id-247206

    Enlarge

  28. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

    Enlarge

  31. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Article
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159149-plasmid-map-sequence-id-310074

    Enlarge

  35. pTT3-ecdCD19-ST3-His_withSUMO

    Plasmid
    #223219
    Purpose
    Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
    Use
    Tags
    Histag, SUMO, and Spytag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    223219-plasmid-map-sequence-id-443124

    Enlarge

  37. pDONR223 SARS-CoV-2 NSP5

    Plasmid
    #141259
    Purpose
    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141259-plasmid-map-sequence-id-288065

    Enlarge

  38. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

    Enlarge

Showing: 8521 - 8560 of 11256 results