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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,426 results for: 11

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Showing: 4261 - 4280 of 7426 results

  3. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  11. pUltraI-Tet3.0[TAA]

    Plasmid
    #164580
    Purpose
    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    Expression
    Bacterial
    Mutation
    L170G, N311G, C313A
    Promoter
    ProK and TacI
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    164580-plasmid-map-sequence-id-392917

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  18. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαs-LgB113 (GNAS Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  19. MT-AvrXa10-FT

    Plasmid
    #234373
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus
    Depositor
    Michael Smanski
    Article
    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202
    Inserts
    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    Use
    Synthetic Biology
    Tags
    GB1, GFP, and GP41
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234373-plasmid-map-sequence-id-469429

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Showing: 4261 - 4280 of 7426 results