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pEPOR2KN0008

Plasmid

#117580

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0004

Plasmid

#117579

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0003

Plasmid

#117578

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0001

Plasmid

#117576

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_25-eGFP

Plasmid

#107413

Purpose

This plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_25

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_7-eGFP

Plasmid

#107412

Purpose

This plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG318-h.72.4d

Plasmid

#99080

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea h.72.4d

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

h.72.4d in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG223-smad2/3

Plasmid

#99078

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Smad2/3

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Smad2/3 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38E

Plasmid

#65665

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38E

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamic acid

Promoter

CMV, 2x Tet

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET:CACT:GFP

Plasmid

#60737

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Bcr del oligo pCDNA3.1/HisB

Plasmid

#36423

Depositor

John Groffen, Nora Heisterkamp

Article

Yi et al BMC Biochem. 2011 Feb 10;12:8.

Insert

BCR (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

Deletion of amino acid residues 2-63

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCW57-GFP-2A-MCS

Plasmid

#71783

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Del 1)

Plasmid

#16601

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 1) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-1 (XmnI->PvuII)

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

Sara Howden, James Thomson

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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