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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159845

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-P2A-CIAR_pLenti_CMV_Puro

Plasmid

#86503

Purpose

Lenti plasmid for generating CIAR expressing stable cell lines

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

GFP-P2A-CIAR (SOS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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sgTP53(R273)

Plasmid

#85561

Purpose

Expresses sgRNA targeting human TP53 around codon R273

Depositor

Luca Grumolato

Publication

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pdCas9 (GB1079)

Plasmid

#75399

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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821 pGEX2T PTEN 1-274

Plasmid

#10741

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-274.

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCas3-Amp

Plasmid

#207998

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Publication

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sCD200

Plasmid

#164424

Purpose

AAV plasmid expressing soluble CD200 in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 40 amino acids

Promoter

Best1

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIIα-Dronpa

Plasmid

#182331

Purpose

PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Publication

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RIIalpha-Dronpa (PRKAR2A Human)

Tags

Full-length human PKA type 2alpha regulatory subu…

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Publication

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBOH-MHL.EED

Plasmid

#125174

Purpose

Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Opto-casp9_VfAU1-LOV_casp9

Plasmid

#131220

Purpose

Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Opto-casp9 (VfAU1-LOV_casp9)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcold-6xHis-HRV3Csite-DmLoqsPA

Plasmid

#41093

Depositor

Phillip Zamore

Publication

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PA (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLPC FLAG hRap1 delta Myb

Plasmid

#13253

Depositor

Titia de Lange

Publication

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Myb (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-131 + 184-399 and the point mu…

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Publication

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

Plasmid

#223213

Purpose

Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Publication

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

TGFb1 active - membrane (TGFB1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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SSpB-iRFP-p63DH

Plasmid

#176120

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-TBP6.9F34MF37MF77M

Plasmid

#168268

Purpose

Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

Depositor

Joseph Wedekind

Publication

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

TBP6.9

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F34M, F37M and F77M

Promoter

T7 promotor

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-H123Y

Plasmid

#28299

Depositor

Maria-Magdalena Georgescu

Publication

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant His123Tyr

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only