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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,310 results for: eng

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Showing: 4281 - 4300 of 14310 results

  1. pQE-BR1

    Plasmid
    #160333
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Publication
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    BR1
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160333-plasmid-map-sequence-id-326604

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  2. pQE80L-SH3

    Plasmid
    #160331
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Publication
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    SH3
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160331-plasmid-map-sequence-id-326603

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  3. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Publication
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218775-plasmid-map-sequence-id-443448

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  6. pcDNA3-sACE2v6-sfGFP

    Plasmid
    #145177
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145177-plasmid-map-sequence-id-292606

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  7. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139991-plasmid-map-sequence-id-275523

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  17. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Publication
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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Showing: 4281 - 4300 of 14310 results