We narrowed to 4,529 results for: gca

1. Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75247

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 mTagBFP2 rat PGK1 shRNA

   Plasmid

   #222869

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat PGK1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

   Plasmid

   #209196

   Purpose

   Mutagenesis of Cnr1

   Depositor

   Larry Zweifel

   Article

   Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

   Insert

   Cnr1 (Cnr1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px330-EGFP-LRRK2-CRISPR/Cas9

   Plasmid

   #180437

   Purpose

   Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   gRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgAPC(B)

   Plasmid

   #85558

   Purpose

   Expresses sgRNA targeting human APC around codon I1417

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   APC, WNT signaling pathway regulator (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBoBi-hLAMP2-C-GC6s

   Plasmid

   #154151

   Purpose

   To detect the calcium ion releases close to the lysosomal surface

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   LAMP2 and GCamp6s (LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Use

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ad:2CMV_A2R2-N25Ctr

    Plasmid

    #122243

    Purpose

    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAGI1 gRNA (BRDN0001146934)

    Plasmid

    #78069

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAGI1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATM gRNA (BRDN0001149518)

    Plasmid

    #77529

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GFAP-eGFP-shAqp4-1

    Plasmid

    #223223

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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