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pD649-HAsp-PSG11-Fc(DAPA)-AviTag-6xHis

Plasmid

#156740

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG11 (PSG11 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH2NLA-Fc(DAPA)-AviTag-6xHis

Plasmid

#156634

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH2NLA (NOTCH2NLA Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SDK2(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157613

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SDK2 (SDK2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ZPBP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156747

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ZPBP (ZPBP Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SDK1(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157615

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SDK1 (SDK1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-PAPLN(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157603

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PAPLN (PAPLN Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

Plasmid

#157388

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG9 (PSG9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM8-COMP5AP-AviTag-9xHis

Plasmid

#157294

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM8 (CEACAM8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NFAM1-COMP5AP-AviTag-9xHis

Plasmid

#157066

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NFAM1 (NFAM1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(T304I)

Plasmid

#177133

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c029

Plasmid

#175471

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

L281R, H288A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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TMD18

Plasmid

#163673

Purpose

Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TMD19

Plasmid

#163674

Purpose

Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TMD20

Plasmid

#163675

Purpose

Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TMD21

Plasmid

#163676

Purpose

Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TMD22

Plasmid

#163677

Purpose

Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TMD24

Plasmid

#163678

Purpose

Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-ST*-GFP

Plasmid

#163669

Purpose

Expresses FKBP tagged partial length ST in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)

Tags

FK506 binding protein (FKBP) and GFP

Expression

Mammalian

Mutation

This chimera contains the first 113 amino acids o…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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