We narrowed to 10,059 results for: Uty

1. SpCas9 TadCBEa

   Plasmid

   #193782

   Purpose

   Express TadCBEa (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa-SpCas9D10A
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmirGLO:Δluc::gfp ΔAmpR::KanR

   Plasmid

   #176640

   Purpose

   MPRAu backbone vector

   Depositor

   Pardis Sabeti

   Article

   Griesemer et al Cell. 2021 Sep 30;184(20):5247-5260.e19.

   Insert

   emGFP
   

   Expression

   Mammalian

   Promoter

   Human PGK-1

   Available Since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

   Plasmid

   #66789

   Purpose

   Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D842V (constitutively active)

   Promoter

   EF1a

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pTPH413

   Plasmid

   #185728

   Purpose

   Bacterial PAM profiling of adenine base editor variants

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Inserts

   ecTadA(8e)-neNme2-C
   Nme2Cas9 sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRISPR-StAR1 (Retro)

   Plasmid

   #222687

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPR-StAR2 (Retro)

   Plasmid

   #222688

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRISPR-StAR3 (Retro)

   Plasmid

   #222689

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PXL

   Plasmid

   #75349

   Purpose

   PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAd-Klf4

    Plasmid

    #19770

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 TadCBEe

    Plasmid

    #193836

    Purpose

    Express TadCBEe (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDe-SpCas9D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNF130 shRNA-TRE

    Plasmid

    #225336

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF130 shRNA (Rnf130 Mouse)
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    TRE (tetracycline-responsive element)

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLXSN-Axl

    Plasmid

    #65222

    Purpose

    Expression of Axl in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

    Insert

    AXL (AXL Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAG225 HIPK1 3'UTR mut

    Plasmid

    #12044

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    HIPK1 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG177 HIPK1 3'UTR mut

    Plasmid

    #12035

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N10 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-ITR-PytR-mCherry

    Plasmid

    #204868

    Purpose

    Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. mazei pyrrolysyl tRNA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    U6

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ITR-EcYtR-mCherry

    Plasmid

    #217362

    Purpose

    Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    E. coli tyrosine tRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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