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ChrV_oxTi365_loxP_myo2_neoR

Plasmid

#194057

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

oxTi365

Expression

Worm

Promoter

None

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMGA-ptet-sfGFP

Plasmid

#139935

Purpose

aTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

TetR-Ptet-sfGFP

Expression

Bacterial

Available Since

July 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET_BGL2

Plasmid

#101911

Purpose

Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

Depositor

Steve Shih

Article

Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

Insert

β-glucosidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7+LacO

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Cyto-GCaMP3

Plasmid

#44331

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

GCaMP3

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-GFP osTIR1

Plasmid

#182514

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pMGB

Plasmid

#139932

Purpose

M. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23101

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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CasRX_Pac

Plasmid

#194803

Purpose

CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

Depositor

Alexander Paredez

Article

Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

Insert

CasRX

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Expression

Worm

Promoter

U6

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Tags

HA

Mutation

um200, no stop codon; N1056S

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Lck-GCaMP3

Plasmid

#44330

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

Lck-GCaMP3

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-5HT

Plasmid

#184597

Purpose

For expression of a fluorescent sensor for serotonin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-5HT

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
JD94

Plasmid

#229479

Purpose

CMV promoter driving expression of adenoviral E2A gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD80

Plasmid

#229480

Purpose

Adenoviral VA RNA I gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA I

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Promoter

UAS

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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