We narrowed to 6,267 results for: cat.2

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.108

Plasmid

#184977

Purpose

Express -Eco1 LYP1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12

Expression

Yeast

Mutation

LYP1 donor E27stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.110

Plasmid

#184979

Purpose

Express -Eco1 TRP2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12

Expression

Yeast

Mutation

TRP2 donor E64stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) M173C

Plasmid

#134696

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

M173C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) F234C

Plasmid

#134697

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

F234C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) V304C

Plasmid

#134698

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

V304C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) I367C

Plasmid

#134699

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

I367C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSC32

Plasmid

#104806

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101090

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC31

Plasmid

#104805

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101090

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC27

Plasmid

#104801

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr4g020620

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC26

Plasmid

#104800

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101040

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC14

Plasmid

#104788

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSC15

Plasmid

#104789

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Expression

Plant

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSC13

Plasmid

#104787

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Expression

Plant

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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p_m16S

Plasmid

#160549

Purpose

Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

Depositor

Denis Gerlier

Article

Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

Insert

16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)

Use

Unspecified

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pBAD-SPW-RHH

Plasmid

#190630

Purpose

Bacterial expression of genetically encoded protease indicator SPW-RHH

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW-RHH

Expression

Bacterial

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCW-HHL

Plasmid

#190634

Purpose

Mammalian expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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