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pAW8_GST

Plasmid

#110237

Purpose

Cre/Lox C-terminal tagging construct encoding glutathione S-transferase

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_6XHis

Plasmid

#110236

Purpose

Cre/Lox C-terminal tagging construct encoding six histidine (His) residues

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_3HA

Plasmid

#110233

Purpose

Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pV238-vPE

Plasmid

#225258

Purpose

Mammalian expression of vPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

vPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pV231-xPE

Plasmid

#225257

Purpose

Mammalian expression of xPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

xPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-puro

Plasmid

#158457

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-blast

Plasmid

#158458

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHM129

Plasmid

#225534

Purpose

Encodes destablized mCherry under AR8 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHM131

Plasmid

#225530

Purpose

Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-TMPRSS11D

Plasmid

#158447

Purpose

Gateway Cloning compatible entry vector for the human TMPRSS11D gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS11D (TMPRSS11D Human)

Use

Gateway entry vector

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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LRRK2_1867_2136

Plasmid

#40408

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867_2136

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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