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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Tags

NLS-3xHA

Expression

Insect

Promoter

act5C

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGL1_4

Plasmid

#199036

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-T-V5-GR-2A-H2BmCherry-IP

Plasmid

#171500

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-ESyn1

Plasmid

#80804

Purpose

hSyn1 promoter fused to CMVmin

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p5E-ESyn1

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - mSRSF6

Plasmid

#120420

Purpose

overexpression of mouse Srsf6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (Srsf6 Mouse)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLK

Plasmid

#64186

Purpose

harbours marker genes LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGL0_4 [pIJ101 ORI]

Plasmid

#198921

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-WspR

Plasmid

#79166

Purpose

Dual expression of Ct biosensor and WspR WT under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

WspR (wspR P. areuginosa)
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMA3735

Plasmid

#184848

Purpose

gRNA cloning using blue-white screening for recombinants

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

LacZalpha

Use

CRISPR

Expression

Mammalian

Mutation

alpha-fragment of LacZ

Promoter

U6

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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