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CMPK2 gRNA (BRDN0001144952)

Plasmid

#75804

Purpose

3rd generation lentiviral gRNA plasmid targeting human CMPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CMPK2 (CMPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMPK2 gRNA (BRDN0001145007)

Plasmid

#75805

Purpose

3rd generation lentiviral gRNA plasmid targeting human CMPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CMPK2 (CMPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMPK2 gRNA (BRDN0001147027)

Plasmid

#75806

Purpose

3rd generation lentiviral gRNA plasmid targeting human CMPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CMPK2 (CMPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CCR5-SZ218a QTY

Plasmid

#162444

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-C chemokine receptor type 5 (CCR5 Human)

Tags

his-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Availability

Academic Institutions and Nonprofits only

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Anti-LRRTM4 [N205B/22R]

Plasmid

#128636

Purpose

Mammalian Expression Plasmid of anti-LRRTM4 (Human). Derived from hybridoma N205B/22.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRRTM4 (Human) recombinant mouse monoclonal antibody (LRRTM4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-AICD-IRES-hrGFP

Plasmid

#107548

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD (APP Human)

Use

AAV

Mutation

None

Promoter

human synapsin

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP.mIKKα

Plasmid

#74413

Purpose

Expresses mouse eGFP-IKKα in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

mouse IKKα (Chuk Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001145502)

Plasmid

#76851

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001148405)

Plasmid

#76852

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001146399)

Plasmid

#76853

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTN gRNA (BRDN0001148106)

Plasmid

#76854

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTN (TTN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MB 94C thsS(t3)R-Bxb1_P7-sfGFP_mCherry

Plasmid

#232471

Purpose

Optimized thiosulfate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

thsS(t3)
thsR
PphsA342
sfGFP
AxeTxe
mCherry
Bxb1 integrase

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Contains the following mutations K286Q, Q350H, I…

Promoter

J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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MB 39Vm13 ttrSR(m13)-bARGSer

Plasmid

#232472

Purpose

Optimized tetrathionate sensor with acoustic reporter genes (bARGSer)

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Tmem119 [L128/18R]

Plasmid

#225367

Purpose

Mammalian Expression Plasmid of anti-Tmem119 (Mouse) IgG2a R-mAb. Derived from hybridoma L128/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Tmem119 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Tmem119 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_FURIN_WT

Plasmid

#82122

Purpose

Gateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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